Analytical Sciences

Abstract − Analytical Sciences, 37(11), 1487 (2021).

Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol
Liyuan MA,* Yao XIAO,* Huajuan FANG,* Hualin YANG,*,** and Yu ZHOU*,***
*College of Life Science, Yangtze University, 266 Jingmi Road, Jingzhou, Hubei 434025, China
**State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil & Water Pollution, Chengdu University of Technology, Chengdu, Sichuan 610059, China
***College of Animal Science, Yangtze University, 266 Jingmi Road, Jingzhou, Hubei, 434025, China
In this work, an alkaline phosphatase (ALP) biosensor was established based on G-quadruplex/N-methylmesoporphyrin IX (G4/NMM) and p-nitrophenol (PNP). Because the absorption of PNP was close to the excitation wavelength of G4/NMM, PNP could reduce the fluorescence of G4/NMM. Meanwhile, PNP was the hydrolysis product of p-nitrophenylphosphate (PNPP) by ALP. Therefore, ALP could be detected. This ALP biosensor had a linear analytical range from 2.5 to 25 U/L a the detection limit of 0.81 U/L. Moreover, it showed a satisfactory selectivity and recovery rates.