Analytical Sciences

Abstract − Analytical Sciences, 36(2), 143 (2020).

Enzyme-responsive Fluorescent Ionic Liquid
Ryoutarou OISHI,* Tatsumi MIZUTA,* Kenji SUEYOSHI,*,** Tatsuro ENDO,*,** and Hideaki HISAMOTO*
*Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka, Sakai, Osaka 599-8531, Japan
**PRESTO, JST, Japan Science Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
Herein, we describe the development of a novel material, “enzyme-responsive fluorescent ionic liquid”, which enabled a highly sensitive detection of alkaline phosphatase (ALP). We prepared a plasticized poly(vinyl chloride) (PVC) membrane using this new material as a plasticizer and quantified ALP in aqueous solutions. Preliminary results suggested that the PVC membrane responded to ALP at an interface between the membrane and the sample solution with anion extraction to maintain electroneutrality in the membrane phase. The developed PVC membrane showed an approximately six-times higher sensitivity than the conventional membrane, thereby demonstrating highly sensitive ALP detection. These results suggested the potential applicability of the proposed membrane for highly sensitive protein detection by using ALP-labeled antibodies.