Analytical Sciences

Abstract − Analytical Sciences, 35(9), 1053 (2019).

Introducing an Experimental Design Approach for Efficient Optimization of Chiral Derivatization Conditions for D- and L-Glyceric Acids
Takahiro TAKAYAMA, Hajime MIZUNO, Toshimasa TOYO’OKA, and Kenichiro TODOROKI
Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan
A sensitive analytical method was developed for individual analyses of D- and L-glyceric acids by chiral derivatization — liquid chromatography–tandem mass spectrometry. To elucidate rapid and efficient optimization for derivatization we newly introduced a concept of design of experiments (DOE). The optimization of major 5 factors in the derivatization could be predicted with only 28 measurements. By applying DOE to optimization, the yields of desired derivatives increased five-fold against before optimization.