Analytical Sciences

Abstract − Analytical Sciences, 35(3), 337 (2019).

On-site Method for Beef Detection Based on Strand Exchange Amplification
Xuejiao WANG,* Chunyu YAN,* Manman WEI,* Chao SHI,** Shuyan NIU,* and Cuiping MA*
*Shandong Provincial Key Laboratory of Biochemical Engineering, College of Chemistry and Molecular Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China
**College of Life Sciences, Qingdao University, Qingdao 266071, PR China
Meat screening plays a significant role in human health and religion. But the identification methods for beef were little reported. In this work, a simple colorimetric method based on denaturation bubble-mediated strand exchange amplification (SEA) was developed for the rapid and sensitive identification of beef. The whole strategy was performed on a portable metal bath and the distinguishable color between positive and negative controls was observed directly by the naked eyes. The feasibility using crude extraction samples by a heating treatment in PBS for 2 min was evaluated in duck spiked by beef. The result demonstrated that the developed method could identify as low as 1% (w/w) beef/duck within 50 min. Meanwhile, the results showed the method had a good repeatability and specificity. Therefore, this assay allows for the rapid, sensitive, specific detection of beef, and can be recommended as an effective, promising strategy for on-site meat identification.