Analytical Sciences

Abstract − Analytical Sciences, 35(2), 223 (2019).

Specific Detection of c-Kit Expressed on Human Cell Surface by Immunosensor Based on Surface Plasmon Resonance
Shiro MIYAKE,*,** Daisuke IRIKURA,** and Tomomi YAMASAKI***
*Department of Food and Life Science, Azabu University, 1-17-71 Fuchinobe, Chuo, Sagamihara, Kanagawa 252-5201, Japan
**Research & Development Division, Horiba, Ltd., 2 Miyanohigashicho, Kisshoin, Minami, Kyoto 601-8510, Japan
***Osaka Institute of Public Health, 1-3-69 Nakamichi, Higashinari, Osaka 537-0025, Japan
An immunosensor based on surface plasmon resonance was developed for detection of c-Kit expressed on a cell surface. The combination of the antibody solution modified with gelatin before immobilization to the sensor chip and its blocking with gelatin drastically decreased the nonspecific reaction. The condition may be useful for the detection of various cells by using antibody against cell surface marker including the c-Kit.