Analytical Sciences

Abstract − Analytical Sciences, 34(3), 355 (2018).

Non-enzymatic Determination of L-Proline Amino Acid in Unprocessed Human Plasma Sample Using Hybrid of Graphene Quantum Dots Decorated with Gold Nanoparticles and Poly Cysteine: A Novel Signal Amplification Strategy
*Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
**Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
***Department of Nano Technology, Faculty of Science, Urmia University, Urmia 57154, Iran
An innovative electrochemical interface for quantitation of L-proline (L-Pro) based on ternary amplification strategy was fabricated. In this work, gold nanoparticles prepared by soft template methodology were immobilized onto green and biocompatible nanocomposite containing poly as a conductive matrix and graphene quantum dots as the amplification element. Therefore, a novel multilayer film based on poly-L-cysteine, graphene quantum dots (GQDs), and gold nanoparticles (GNPs) was exploited to develop a highly sensitive electrochemical sensor for the detection of L-Pro. Fully electrochemical methodology was used to prepare a new transducer on a glassy carbon electrode, which provided a high surface area towards sensitive detection of L-Pro. The prepared electrode was employed for the detection of L-Pro. Under optimized conditions, the calibration curve for L-Pro concentration was linear in 0.5 nM – 10 mM with a low limit of quantification of 0.1 nM. The practical analytical utility of the modified electrode was illustrated by determination of L-Pro in unprocessed human plasma samples.