Analytical Sciences

Abstract − Analytical Sciences, 34(10), 1201 (2018).

Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish
Zhen CHEN,*1 Liqing ZANG,*2 Yue WU,*1 Hiroko NAKAYAMA,*2 Yasuhito SHIMADA,*3,*4 Rojeet SHRESTHA,*1 Yaoyao ZHAO,*1 Yusuke MIURA,*1 Hitoshi CHIBA,*5 Shu-Ping HUI,*1 and Norihiro NISHIMURA*2
*1 Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita, Sapporo 060-0812, Japan
*2 Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan
*3 Department of Integrative Pharmacology, Graduate School of Medicine, Mie University, Tsu, Mie 514-8507, Japan
*4 Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan
*5 Department of Nutrition, Sapporo University of Health Sciences, Nakanuma Nishi-4-2-1-15, Higashi, Sapporo 007-0894, Japan
Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies.