Abstract − Analytical Sciences, 33(9), 989 (2017).
Time-resolved Fluorescent Detection for Glucose Using a Complex of Luminescent Layered Titanates and Enzymes
Yuki SAKAGUCHI,* Tomoka MINAMIKAWA,* Mayuko YAMAMURO,* Tadayuki TSUJITA,* Toshihisa UEDA,* Kai KAMADA,** and Nobuaki SOH*
*Department of Biochemistry and Food Science, Faculty of Agriculture, Saga University, 1 Honjyo-machi, Saga 840-8502, Japan
**Department of Chemistry and Materials Engineering, Graduate School of Engineering, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
**Department of Chemistry and Materials Engineering, Graduate School of Engineering, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
Luminescent europium-doped layered titanates (Eu-TiOx) were synthesized and complexed with horseradish peroxidase (HRP) and glucose oxidase (GOx). The emission of a resultant Eu-TiOx/HRP/GOx complex decreased upon the addition of glucose in the presence of guaiacol. The emission decrease was dependent on the concentrations of glucose, and the detection limit for glucose was 3.1 μM. The proposed system would be promising as a new detection method for glucose.
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