Analytical Sciences

Abstract − Analytical Sciences, 33(7), 845 (2017).

Diffusion-controlled Mediated Electron Transfer-type Bioelectrocatalysis Using Microband Electrodes as Ultimate Amperometric Glucose Sensors
Yukina MATSUI, Katsumi HAMAMOTO, Yuki KITAZUMI, Osamu SHIRAI, and Kenji KANO
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kitashirakawaoiwake-cho, Sakyo, Kyoto 606-8502, Japan
We performed numerical simulations on an extremely fast, mediated, electron transfer-type bioelectrocatalytic reaction using a microband electrode. The simulations under fast-enzyme-kinetics conditions predicted that the decrement of the current density by increaseing the microband thickness would effectively improve the upper limit of detection. These predictions were accurate for an ultrathin-ring with thickness of 100 nm and gold leaf with thickness of 10 μm electrodes, acting as novel amperometric glucose sensors with FAD-dependent glucose dehydrogenase. The gold leaf electrode provided pseudo-steady-state currents which were proportional to the glucose concentration up to a concentration of 20 times higher than the mediator concentration.