Analytical Sciences

Abstract − Analytical Sciences, 33(6), 735 (2017).

Nanostructures Integrated with a Nanochannel for Slowing Down DNA Translocation Velocity for Nanopore Sequencing
Xiaoyin SUN,*1,*2 Takao YASUI,*1,*2,*3 Takeshi YANAGIDA,*4,*5 Noritada KAJI,*1,*2,*3 Sakon RAHONG,*1,*2 Masaki KANAI,*4 Kazuki NAGASHIMA,*4 Tomoji KAWAI,*5 and Yoshinobu BABA*1,*2,*6
*1 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8603, Japan
*2 ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa, Nagoya 464-8603, Japan
*3 PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
*4 Institute of Materials Chemistry and Engineering, Kyushu University, 6-1 Kasuga-Koen, Kasuga, Fukuoka 816-8580, Japan
*5 Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka-cho, Ibaraki, Osaka 567-0047, Japan
*6 Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan
Here, we developed a device integrated with a nanochannel and nanostructures to slow DNA translocation velocity. We found that translocation velocity of a single DNA molecule inside a nanochannel was decreased by pre-elongating it using some nanostructures, such as a shallow channel or nanopillars. This decrease of the translocation velocity was associated with the DNA mobility change, which is an intrinsic parameter of DNA molecules and unaffected by an electric field.