Analytical Sciences

Abstract − Analytical Sciences, 33(11), 1237 (2017).

Detection of Scopolamine Hydrobromide via Surface-enhanced Raman Spectroscopy
Lin BAO,*,** Xuan-Yu SHA,* Hang ZHAO,* Si-qin-gao-wa HAN,*** and Wu-Li-Ji HASI*
*National Key Laboratory of Science and Technology on Tunable Laser, Harbin Institute of Technology, Harbin 150080, China
**College of Physics and Electronics Information Inner Mongolia University for Nationalities, Inner Mongolia Tongliao 028043, China
***Affiliated Hospital, Inner Mongolia University for the Nationalities, Inner Mongolia Tongliao 028007, China
Surface-enhanced Raman spectroscopy (SERS) was used to measure scopolamine hydrobromide. First, the Raman characteristic peaks of scopolamine hydrobromide were assigned, and the characteristic peaks were determined. The optimal aggregation agent was potassium iodide based on a comparative experimental study. Finally, the SERS spectrum of scopolamine hydrobromide was detected in aqueous solution, and the semi-quantitative analysis and the recovery rate were determined via a linear fitting. The detection limit of scopolamine hydrobromide in aqueous solution was 0.5 μg/mL. From 0 – 10 μg/mL, the curve of the intensity of the Raman characteristic peak of scopolamine hydrobromide at 1002 cm−1 is y = 4017.76 + 642.47x. The correlation coefficient was R2 = 0.983, the recovery was 98.5 – 109.7%, and the relative standard deviation (RSD) was about 5.5%. This method is fast, accurate, non-destructive and simple for the detection of scopolamine hydrobromide.