Analytical Sciences

Abstract − Analytical Sciences, 32(8), 853 (2016).

Electrochemical Sensing of Casein Based on the Interaction between Its Phosphate Groups and a Ruthenium(III) Complex
Iku INABA,* Hideki KURAMITZ,** and Kazuharu SUGAWARA*
*Maebashi Institute of Technology, 460-1 Kamisadori, Maebashi, Gunma 371-0816, Japan
**Department of Environmental Biology and Chemistry, Graduate School of Science and Engineering for Research, University of Toyama, 3190 Gofuku, Toyama, Toyama 930-8555, Japan
A reaction to casein, along with β-lactoglobulin, is a main cause of milk allergies, and also is a useful indicator of protein in allergic analyses. In the present study, a simple casein sensor was developed based on the interaction between a phosphate group of casein and electroactive [Ru(NH3)6]3+. We evaluated the voltammetric behavior of a casein-[Ru(NH3)6]3+ complex using a glassy carbon electrode. When the ruthenium(III) complex was combined with the phosphate groups of casein, the structure of the casein was changed. Since the hydrophobicity of casein was increased due to the binding, the casein was adsorbed onto the electrode. Furthermore, we modified an electrode with a ruthenium(III) ions/collagen film. When the sensor was applied to the detection of the casein contained in milk, the values coincided with those indicated by the manufacturer. Accordingly, this electrode could be a powerful sensor for the determination of casein in several foods.