Analytical Sciences

Abstract − Analytical Sciences, 32(5), 523 (2016).

Fluorescence Detection of DNA Based on Non-covalent π-π Stacking Interaction between 1-Pyrenebutanoic Acid and Hypericin
Weiwen HU,* Jian ZHANG,** and Jinming KONG*
*School of Environmental and Biological Engineering, Nanjing University of Science & Technology, Nanjing 210094, P. R. China
**Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, Jiangsu Province, P. R. China
Herein, we applied hypericin (Hyp) for the detection of DNA on streptavidin-functionalized magnetic beads (SA-MB) through non-covalent π-π stacking interaction between 1-pyrenebutanoic acid (PBA) and Hyp. Briefly, the target DNA (tDNA) was hybridized to morpholino oligonucleotide (MO) that was immobilized on the surface of SA-MB. PBA was incorporated to the backbone of tDNA by means of phosphate-zirconium-carboxylate coordination reaction. Then, fluorescent Hyp was linked to PBA via π-π stacking interaction. Under the optimal conditions, this biosensor displayed a good linear relationship between the fluorescence intensity and the tDNA concentrations in the range from 1 to 100 nM with a low detection limit of 2.9 nM. The mismatch type in a specific DNA sequence can also be efficiently discriminated. Compared with some other reports, this method is more convenient, and has great potential for practical applications.