Analytical Sciences

Abstract − Analytical Sciences, 32(5), 487 (2016).

Purification of Pyridylaminated Oligosaccharides Using 1,2-Dichloroethane Extraction
Yusuke SUZUKI,* Aya OKANO,* Kazuya KABAYAMA,** Atsuyoshi NISHINA,* Minoru TANIGAWA,* Katsushi NISHIMURA,* and Yasunori KUSHI*
*College of Science and Technology, Nihon University, 228A College of Sci. and Tech. Bldg. No. 2, 1-5-1 Kanda Surugadai, Chiyoda, Tokyo 101-0062, Japan
**Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan
Fluorescence derivatization of the oligosaccharides released from glycoconjugates is widely used for precise structural characterization. To ensure labeling of the oligosaccharides, a large excess of fluorescence reagents is usually added to the reaction tube. Therefore, any excess reagents and by-products of the labeling reaction should be removed by several column chromatographies, including using a cellulose cartridge or spin columns. However, these purification steps are often time-consuming, expensive, and laborious. In this study, we found that 1,2-dichloroethane extraction could effectively and easily purify pyridylaminated oligosaccharides with a high recovery rate.