Analytical Sciences

Abstract − Analytical Sciences, 32(10), 1035 (2016).

One-pot Synthesis of Quencher Labeled Hairpin DNA–CdTe QDs Conjugate for Target DNA and Deoxyribonuclease I Detection
Qingfeng ZHANG,*,** Juanmin LI,*,** Yunyi ZHANG,*,**,*** Zhenzhen HU,*,** and Cong YU*,**
*State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625, Renmin Street, Changchun 130022, P. R. China
**University of Chinese Academy of Sciences, Beijing 100049, P. R. China
***School of Materials Science and Engineering, Tianjin University, Tianjin 300072, P. R. China
A black-hole quencher (BHQ-2) labeled DNA (Q-DNA) with a phosphorothioate backbone was covalently conjugated to the CdTe QDs during the QDs synthesis procedure. The hairpin structure of Q-DNA shortened the distance of the CdTe QDs and the BHQ-2 group, which resulted in fluorescence quenching of the QDs. The addition of target DNA or deoxyribonuclease I (DNase I) could move the BHQ-2 group away from the QDs. As a result, the fluorescence of the CdTe QDs recovered. This work provides a new way for target DNA and DNase I detection.