Analytical Sciences

Facile Synthesis of Glutathione-capped CdS Quantum Dots as a Fluorescence Sensor for Rapid Detection and Quantification of Paraquat

Hongxia LI,* Jingjing LIU,** and Xiaohong YANG*

*School of Pharmacy, Jilin University, Changchun 130021, P. R. China
**Department of Gastrointestinal Surgery, Second Hospital of Jilin University, Changchun 130041, P. R. China

This paper describes a convenient and rapid fluorescence sensor for determination of paraquat (PA) based on glutathione-capped CdS quantum dots (QDs). The methodology enabled the use of a simple synthesis procedure for water solubilization of CdS QDs via a fast route using glutathione as a capping agent within 15 min. The resulting water-soluble QDs exhibit a strong fluorescence emission at 536 nm with high and reproducible photostability. PA is an important class of electron acceptors for QDs. Thus, the fluorescence intensity of the glutathione-capped CdS QDs probe could be dramatically quenched by PA due to the electron transfer mechanism. The fluorescence intensity of the CdS QDs system was proportional to PA concentration in the range of 0.025 to 1.5 μg mL⁻¹, with a detection limit of 0.01 μg mL⁻¹. The time of analysis sample, including preparation of QDs and fluorescent measurement for PA, was only 20 min. Most of the potentially coexisting substances did not interfere with the PA-induced quenching effect except diquat. Furthermore, the analytical applicability of the proposed method was demonstrated by analyzing PA in water, rice and cabbage samples, and the recoveries were between 86 and 105% which satisfied the requirement of detection for PA. These results showed that the proposed method was simple in design and fast in operation, and could be used as a sensitive tool for detecting PA in environmental and agricultural samples.

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