Analytical Sciences

Abstract − Analytical Sciences, 29(4), 393 (2013).

Pretreatment Method for Immunoassay of Polychlorinated Biphenyls in Transformer Oil Using Multilayer Capillary Column and Microfluidic Liquid–Liquid Partitioning
Arata AOTA,* Yasumoto DATE,*,** Shingo TERAKADO,* and Naoya OHMURA*
*Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko, Chiba 270-1194, Japan
**Graduate School of Environmental Studies, Tohoku University, 6-6-11 Aramaki, Aoba, Sendai, Miyagi 980-8579, Japan
Polychlorinated biphenyls (PCBs) are persistent organic pollutants that are present in the insulating oil inside a large number of transformers. To aid in eliminating PCB-contaminated transformers, PCBs in oil need to be measured using a rapid and cost-effective analytical method. We previously reported a pretreatment method for the immunoassay of PCBs in oil using a large-scale multilayer column and a microchip with multiple microrecesses, which permitted concentrated solvent extraction. In this paper, we report on a more rapid and facile pretreatment method, without an evaporation process, by improving the column and the microchip. In a miniaturized column, the decomposition and separation of oil were completed in 2 min. PCBs can be eluted from the capillary column at concentrations seven-times higher than those from the previous column. The total volume of the microrecesses was increased by improving the microrecess structure, the enabling extraction of four-times the amount of PCBs achieved with the previous system. By interfacing the capillary column with the improved microchip, PCBs in the eluate from the column were extracted into dimethyl sulfoxide in microrecesses with high enrichment and without the need for evaporation. Pretreatment was completed within 20 min. The pretreated oil was analyzed using a flow-based kinetic exclusion immunoassay. The limit of detection of PCBs in oil was 0.15 mg kg−1, which satisfies the criterion set in Japan of 0.5 mg kg−1.