Analytical Sciences

Abstract − Analytical Sciences, 29(12), 1183 (2013).

Simultaneous Determination of Azoles Antifungal Drugs in Chicken Tissues by Ultra-performance Liquid Chromatography Tandem Mass Spectrometry
Nan SHANG,*,** Jing ZHANG,* and Bing SHAO*
*Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, P. R. China
**Department of Pharmacy, First Hospital of Shanxi Medical University, Taiyuan 030001, P. R. China
An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method was established for the simultaneous determination of residues of enilconazole, parconazole, thiabendazole and its metabolite, 5-hydroxythiabendazole, in chicken tissues including liver, muscle and egg. Homogenized samples were extracted by acetonitrile, concentrated and purified by an Oasis MCX solid-phase extraction cartridge. Analysis of the target drugs was performed by liquid chromatography with an UPLCTM BEH C18 column, coupled with a tandem mass spectrometry (UPLC-MS/MS) operating in the positive multiple-reaction mode (MRM). Good linearity was obtained at concentrations of 0.10 – 100 μg kg−1. The average recoveries of the analytes in chicken liver, muscle and egg at six fortification levels were in the range 93.6 – 119.5% with acceptable coefficients of variation (<14.8%). The detection capability (CCβ) of the analytes upon the method ranged from 0.05 to 138.1 μg kg−1. This method was successfully applied in screening and confirming of target drugs in tens of real samples.