Analytical Sciences

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

Toru TAKAHASHI,* Jun KAWANA,** Yuuki TAMURA,** and Hitoshi HOSHINO**

*Graduate School of Engineering, University of Fukui, 3-9-1 Bunkyo, Fukui 910-8507, Japan
**Graduate School of Environmental Studies, Tohoku University, 20 Aoba, Aramaki, Aoba, Sendai 980-8579, Japan

A simple, rapid, and precise dynamic coating capillary electrophoretic separation method for water-soluble humic substances is proposed. An aqueous solution containing hydroxyethyl cellulose (HEC), polyethylene glycol (PEG), and pH buffer component was employed for both the dynamic coating agent and the separation medium. The procedure for the coating of the capillary inner wall was simply filling the buffered polymer mixture solution into the capillary that had been treated with 1 M aqueous HCl solution. The solution for the capillary coating was directly used as the electrophoretic buffer solution for CE separation. Excellent performance for the separation of humic acid was obtained using the solution containing 0.5% (w/v) HEC, 1.0% (w/v) PEG 10000, and 0.1% (w/v) PEG 8000000. Excellent reproducibility and durability were obtained even at slightly alkaline conditions at pH levels above 8. The separation of 0.1 – 2 kbp of DNA ladder by the proposed method showed was also achieved.

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