Analytical Sciences


Abstract − Analytical Sciences, 28(6), 613 (2012).

Light Scattering: a Novel Approach to Analyze Protein 4.1R FERM Domain Interaction with Inside-out-vesicles in Solution
Kohei SHIBA,*1 Wataru NUNOMURA,*2,*3 and Yuichi TAKAKUWA*4
*1 Sysmex Corporation, 4-4-4 Takatsukadai, Nishi, Kobe 651-2271, Japan
*2 Center for Geo-Environmental Science, Graduate School of Engineering and Resource Science, Akita University, 1-1 Tegata-Gakuén, Akita 010-8502, Japan
*3 Department of Life Science, Graduate School of Engineering and Resource Science, Akita University, 1-1 Tegata-Gakuén, Akita 010-8502, Japan
*4 Department of Biochemistry, Tokyo Women’s Medical University, 8-1 Kawada, Shinjuku, Tokyo 162-8666, Japan
In this study, we describe a novel application for light scattering, a method widely used for separation of molecules in solution based on their size. We demonstrate that light scattering analysis can monitor the change in particle size of protein 4.1R prior to and after binding to red blood cell inside-out-vesicles in solution. Light scattering constitutes therefore a novel tool to analyze protein-binding association constants.