Analytical Sciences

Gold Nanoparticles as Localization Markers for Direct and Live Imaging of Particle Absorption through a Caco-2 Cell Monolayer Using Dark-Field Microscopy

Toshiro KOBORI,* Jun WATANABE,* and Hidenobu NAKAO**

*National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kan-nondai, Tsukuba 305–8642, Japan
**National Institute of Materials Science, 1-1 Namiki, Tsukuba 305–0044, Japan

Recently, efforts have been made to reduce the size of food particles containing functional ingredients, since reducing the size is expected to improve intestinal absorption. However, the absorption mechanisms have yet to be fully clarified. Therefore, a microscopy-based method for studying interactions between the particles and intestinal cells is required. We optimized the experimental conditions for observing gold nanoparticles (AuNPs) on the surface of an unfixed Caco-2 cell using dark-field microscopy (DFM). Tight junctions were clearly visible with AuNPs on the cells, producing intense scattered light under DFM. This suggests that AuNPs could be used as localization markers to visualize particle absorption through Caco-2 cells.

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