Analytical Sciences

Abstract − Analytical Sciences, 27(9), 957 (2011).

Establishment of a Confluent Cardiomyocyte Culture in a Cylindrical Microchannel
Yo TANAKA,*,**,*** Hiroto AKAIKE,* Yasuhiko SUGII,*,*** and Takehiko KITAMORI*,***
*Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan
**Quantitative Biology Center (QBiC), RIKEN, 2-2-3 Minatojima-Minamimachi, Chuo, Kobe 650-0047, Japan
***Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 1-1-2 Otemachi, Chiyoda, Tokyo 100-0004, Japan
We established a confluent cardiomyocyte culture method using an 800-μm diameter cylindrical microchannel in this report. This was realized by introducing cardiomyocytes 2 times before and after turning over a microchip. The optimum condition was starting the flowing medium 2.0 h after seeding and flowing the medium at 1.0 μL/min. By applying this technology to a cardiomyocyte-based spherical heart pump device, one may develop self-fluid regulated devices that could be applied for implantable or circulation analysis device on a chip.