Abstract − Analytical Sciences, 27(6), 663 (2011).
Simultaneous Determination of Dextromethorphan and Its Metabolite Dextrorphan in Plasma Samples Using Second-order Calibration Coupled with Excitation-Emission Matrix Fluorescence
Ya-Juan LIU, Hai-Long WU, Jin-Fang NIE, Jian-Yao WANG, Li-Qun OUYANG, and Ru-Qin YU
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Dextromethorphan (DEX) is an antitussive agent used in many cough and cold medications, and dextrorphan (DOR) is its metabolite. Owing to their similar structures, optimization of the condition for the chromatography approach, which is in common use for determination, is both demanding and time-consuming. This paper describes a methodology that combines excitation-emission matrix fluorescence spectra with second-order calibration, and was applied to simultaneously and directly determine DEX and DOR contents in plasma samples.
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