Analytical Sciences

Functional Preconcentration Tip of Total Volume Injection for ESI/MS Analysis of DNA Adducts

Hiroya MURAKAMI,* Mio KOGUCHI,* Yukihiro ESAKA,* Bunji UNO,* and Yasushi ISHIHAMA**

*Gifu Pharmaceutical University, Daigaku-nishi, Gifu 501-1196, Japan
**Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida-Shimo-Adachi, Sakyo, Kyoto 606-8501, Japan

We have developed a simple method to significantly improve the sensitivity in the LC/MS analysis of DNA adducts. A preconcentration tip for the selective recovery of DNA adducts was prepared. Using this tip, the total amount of DNA adducts in a treated DNA sample was injected in a one-shot manner into an LC/MS system. We were able to improve the sensitivity by more than one order of magnitude in concentration. This method will be a useful tool for the quantitative determination of trace DNA adducts.

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