Analytical Sciences

Moment Analysis of Chromatographic Behavior of Superficially Porous Particles

Kanji MIYABE,* Kazuhiro NOIRI,** and Kouki KOBAYASHI**

*Graduate School of Science and Engineering for Research, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan
**Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan

Peak parking experiments were conducted to study the chromatographic behavior in a RPLC system consisting of a column packed with superficially porous C₁₈-particles and a mixture of methanol and water (70/30, v/v). The values of the surface diffusion coefficient and the retention equilibrium constant of a column packed with superficially porous C₁₈-particles were comparable to those of columns packed with a C₁₈-silica monolith and full-porous C₁₈-silica gel particles. The flow-rate dependence of HETP was hypothetically calculated by using moment equations to clarify the influence of the structural characteristics on the chromatographic behavior. The column efficiency of a column packed with the superficially porous particles is higher in the high flow-rate range than that with full-porous spherical particles. This is attributed to the smaller contribution of the intraparticulate mass transfer in the superficially porous particles to band broadening. The moment equations are effective for the quantitative analysis of chromatographic behavior of superficially porous particles.

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