Analytical Sciences

Abstract − Analytical Sciences, 26(9), 943 (2010).

Enantiomeric Separation by Capillary Electrochromatography on a Sulfated Poly β-Cyclodextrin Modified Silica-based Monolith
Ruijuan YUAN,* Yan WANG,** and Guosheng DING**
*School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, P. R. China
**School of Pharmaceutical Science & Technology, Tianjin University, Tianjin 300072, P. R. China
A sulfated poly β-cyclodextrin (SPCD) modified silica-based monolithic column was prepared for enantiomeric separation. First, 2-hydroxy-3-allyloxy-propyl-β-cyclodextrin (allyl-β-CD) was bonded onto a bifunctional reagent 3-(methacryloxy)propyltriethoxysilane (γ-MAPS) modified silica-based monolith through radical polymerization; the column was then sulfated with chlorosulfonic acid. The SPCD chiral stationary phase resolved the boring problem associated with desalting when sulfated CDs were synthesized to act as chiral additives. The inorganic salt in the column introduced during the sulfating process could be easily removed by washing the column with water for some time. Chiral compounds investigated were successfully resolved into their enantiomers on the SPCD modified monolith in the capillary electrochromatography (CEC) mode. Due to the existence of the −SO3H group, electrosmotic flow (EOF) was obviously increased, and all of the separations could be carried out in 20 min with only a minor loss in the column efficiency and resolution.