Analytical Sciences


Abstract − Analytical Sciences, 26(8), 891 (2010).

Simultaneous Determination of Albendazole and Praziquantel by Second Derivative Spectrophotometry and Multivariated Calibration Methods in Veterinary Pharmaceutical Formulation
César SOTO,* David CONTRERAS,* Sandra ORELLANA,** Jorge YAÑEZ,* and M. Inés TORAL**
*Department of Analytical and Inorganic Chemistry, Faculty of Chemical Sciences, University of Concepción, P. O. Box 160, Concepción, Chile
**Department of Chemistry, Faculty of Sciences, University of Chile, P. O. Box 653, Santiago, Chile
The simultaneous determination of albendazole (ABZ) and praziquantel (PZQ) was performed by different mathematical approaches: second derivative spectrophotometry (SDS), classical least squares, regression of partial least squares and principal components regression based on spectral data of drugs dissolved in methanol-hydrochloric acid solution. The detection limits for multivariate calibrations were determined by creating a surrogate variable signal. SDS presented the best analytical features. The recoveries of ABZ and PZQ from the synthetic samples were near to 100 ± 5%. The methods were applied in veterinary pharmaceutical formulation whose mass ratio ABZ:PZQ is 10:1; the results obtained were according to nominal content.