Analytical Sciences

Abstract − Analytical Sciences, 26(6), 645 (2010).

Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal
Md Towhid HOSSAIN,* Takayuki SHIBATA,* Tsutomu KABASHIMA,* and Masaaki KAI*,**
*Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan
**Global Center of Excellence Program, Nagasaki University, Nagasaki 852-8521, Japan
Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot−1.