Analytical Sciences

Abstract − Analytical Sciences, 26(6), 641 (2010).

Separation of Optical Isomers in Capillary Chromatography Using a Poly(tetrafluoroethylene) Capillary Tube and an Aqueous–Organic Mixture Carrier Solution
Seiji ISHIMOTO, Naoya JINNO, Masahiko HASHIMOTO, and Kazuhiko TSUKAGOSHI
Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan
Capillary chromatography for the separation of optical isomers was developed using an untreated poly(tetrafluoroethylene) capillary tube and a water–hydrophilic/hydrophobic organic solvent mixture as a carrier solution. The open tubular capillary was 110 cm in length (90 cm effective length) and 100 μm in inner diameter. The carrier solution was prepared with a water–acetonitrile–ethyl acetate mixture (15:3:2 volume ratio) containing 1 mM β-cyclodextrin. A model analyte solution of dansyl-DL-methionine was injected into the capillary tube by a gravity method. The analyte solution was subsequently delivered through the capillary tube with the carrier solution by a microsyringe pump; the system worked under laminar-flow conditions. The analytes were separated through the capillary tube with on-capillary detection by an absorption detector. D-Isomer and L-isomer were eluted in this order with the water-acetonitrile–ethyl acetate carrier solution including β-cyclodextrin.