Analytical Sciences

Abstract − Analytical Sciences, 26(12), 1305 (2010).

Determination of Cyanuric Acid in Milk Powder by Anion-exchange Chromatography
Hui WANG,* Shengjie HOU,* Mingyu DING,* and Jinghua ZHANG**
*Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
**Beijing Center for Physical and Chemical Analysis, Beijing 100089, P. R. China
An anion-exchange chromatographic method has been developed for simultaneous analysis of cyanuric acid (CA) and five inorganic anions (F, Cl, NO3, HPO42− and SO42−) in milk powder. The separation was achieved within 15 min on an anion-exchange column with simple elution of Na2CO3/NaHCO3 buffer as mobile phase. Furthermore, the effect of the total concentration of Na2CO3/NaHCO3 buffer on the retention of five inorganic anions was more obvious than that on CA retention, which indicated that CA retention on anion-exchange column depends not only on anion-exchange interaction but also on hydrophobic interactions between CA and anion-exchange column. The linear range of the calibration curve for CA was 0.1 – 100 mg L−1. The detection limit calculated at S/N = 3 was 0.083 mg L−1. The method was successfully applied to the analysis of CA in milk powder.