Analytical Sciences

Abstract − Analytical Sciences, 26(12), 1277 (2010).

1H NMR-based Metabonomic Analysis of Metabolic Changes in Streptozotocin-induced Diabetic Rats
Liangcai ZHAO,* Xia LIU,* Liyun XIE,** Hongchang GAO,** and Donghai LIN*,***
*Shanghai Institute of Materia Medica, The Chinese Academy of Sciences, Shanghai 201203, P. R. China
**School of Pharmacy, Analysis and Testing Center, Wenzhou Medical College, Wenzhou 325035, P. R. China
***The Key Laboratory for Chemical Biology of Fujian Province, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, P. R. China
Diabetes mellitus is a complex metabolic disorder characterized by chronic hyperglycemia, hypoinsulinemia, and ketosis. To access the biochemical process of diabetes, we applied quantitative 1H NMR-based metabonomics to analyze urine, serum, and liver extracts from streptozotocin-induced diabetic rats. Principle component analysis (PCA) of 1H NMR spectra disclosed metabolic pattern differences between diabetic and control rats, and identified the related metabolic changes. The PCA scores plot demonstrated that the diabetic group could be distinguished from the control group, indicating that the metabolic characteristics of the two groups were markedly different. Our work reveals the accumulation of triglycerides, fatty acids and acetoacetate in diabetic rats, and may provide an efficient, convenient way for evaluating the pathological state and biochemical process of diabetes mellitus.