Analytical Sciences


Abstract − Analytical Sciences, 26(11), 1163 (2010).

Application and Equivalence Assessment for Determining Ethamsylate by Using Potassium Ferricyanide as Spectroscopic Probe Reagent
Litao LIU, Jing LI, and Quanmin LI
College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, P. R. China
In this paper, a novel method has been established to determine ethamsylate using potassium ferricyanide as a spectroscopic probe reagent. It has been demonstrated that Fe(III) is reduced to Fe(II) by ethamsylate, and that the formed Fe(II) reacts with potassium ferricyanide to form soluble prussian blue (KFeIII[FeII(CN)6]). Beer’s law is obeyed in the range of 0.16 – 24.00 μg mL−1 with the molar absorption coefficient of 2.1 × 104 L mol−1 cm−1. The detection limit (3 σ/k) is 0.11 μg mL−1. This method has been successfully applied to determine ethamsylate in pharmaceutical and serum samples with satisfactory results, and presented quite satisfactory credibility during method equivalence assessment.