Analytical Sciences

Abstract − Analytical Sciences, 25(7), 931 (2009).

High-Performance Liquid-Chromatographic Separation of Ofloxacin Using a Chiral Stationary Phase
Xiaojie SUN,* Di WU,** Bing SHAO,* and Jing ZHANG*
*Central Laboratory, Beijing Center for Disease Prevention & Control, Beijing 100013, China **College of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China
A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm × 4.6 mm, 5 μm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers.