Analytical Sciences

Abstract − Analytical Sciences, 24(7), 907 (2008).

Gold Nanoparticle-based Surface Enhanced Raman Scattering Spectroscopic Assay for the Detection of Protein-Protein Interactions
Tao LI,*,** Liping GUO,** and Zhenxin WANG*
*State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
**School of Chemistry, Northeast Normal University, Changchun 130022, China
In the present work, a sensitive spectroscopic assay based on surface-enhanced Raman spectroscopy (SERS) using gold nanoparticles as substrates was developed for the rapid detection protein-protein interactions. Detection is achieved by specific binding biotin-modification antibodies with protein-stabilized 30 nm gold nanoparticles, followed by the attachment of avidin-modification Raman-active dyes. As a proof-of-principle experiment, a well-known biomolecular recognition system, IgG with protein A, was chosen to establish this new spectroscopic assay. Highly selective recognition of IgG down to 1 ng/ml in solution has been demonstrated.