Analytical Sciences

Abstract − Analytical Sciences, 24(2), 181 (2008).

Nuclease Tolerant FRET Probe Based on DNA-Quantum Dot Conjugation
Daisuke ONOSHIMA,*1,*2 Noritada KAJI,*1,*2 Manabu TOKESHI,*1,*2 and Yoshinobu BABA*1,*2,*3,*4,*5
*1 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan
*2 MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Nagoya 464-8603, Japan
*3 Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan
*4 Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan
*5 Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki 444-8585, Japan
We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).