Analytical Sciences

Spectrophotometric Determination of Spermine and Related Compounds Using o-Hydroxyhydroquinonephthalein and Manganese(II)

Kanako MIYACHI,* Kenzo MORIYAMA,* Takako YAMAGUCHI,** Hiroshi TOMINAGA,** Shinichiro KAMINO,** and Yoshikazu FUJITA**

*Department of Hospital Pharmacy School of Medicine, Kinki University, 377-2, Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan
**Osaka University of Pharmaceutical Sciences, 4-20-1, Nasahara, Takatsuki, Osaka 569-1094, Japan

A simple and highly sensitive spectrophotometric method for the determination of spermine (Spm) was established based on the ternary complex formation reaction of Spm with o-hydroxyhydroquinonephthalein (QP) as a xanthene dye and manganese(II) as a metal ion in the presence of a dispersion agent. The apparent molar absorptivity at 555 nm and the relative standard deviation of the proposed method were 1.4 × 10⁵ dm³ mol^-1 cm^-1 and 0.50% (n = 10), respectively. In the method for flow-injection analysis (FIA), which employs a single-channel flow manifold system, a good linear relationship was observed over the 2 - 20 pg µl^-1 range of Spm by direct injection.

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