Abstract − Analytical Sciences, 23(8), 1015 (2007).
Studies on Neurosteroids XXI: An Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains
Tatsuya HIGASHI, Tadashi NISHIO, Hiroyuki YOKOI, Yasuaki NINOMIYA, and Kazutake SHIMADA
Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method for the determination of the rat brain 5α-androstane-3α,17β-diol (3α,5α-Adiol) has been developed and validated. The brain extract was purified using solid-phase extraction cartridges, derivatized with isonicotinoyl azide, and subjected to LC-MS/MS. The method was accurate and reproducible, and the limit of quantitation was 0.1 ng/g tissue when a 100-mg tissue sample was used. The change in the brain 3α,5α-Adiol level by immobilization stress was also analyzed using the developed method.
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