Analytical Sciences


Abstract − Analytical Sciences, 23(2), 183 (2007).

Determination of Atenolol and Metoprolol by Capillary Electrophoresis with Tris(2,2′-bipyridyl)ruthenium(II) Electrochemiluminescence Detection
Jianshe HUANG, Jinying SUN, Xiaoguang ZHOU, and Tianyan YOU
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China
Capillary electrophoresis (CE) coupling with a tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) electrochemiluminescence (ECL) detection technique was developed for the analysis of two β-blockers, atenolol (AT) and metoprolol (ME). The parameters that influence the separation and detection, including the buffer pH and concentration, the separation voltage, the detection potential and Ru(bpy)32+ concentration, were optimized in detail. The calibration curve was linear over a concentration range of two or three orders of magnitude for the two β-blockers. The detection limits for AT and ME were 0.075 and 0.005 µM (S/N = 3). The relative standard deviations (n = 8) of the ECL intensity and the migration time were 2.65 and 0.22% for AT, 2.82 and 0.34% for ME, respectively. The proposed method was applied to determine AT and ME in spiked urine samples; satisfactory results were obtained.