Analytical Sciences


Abstract − Analytical Sciences, 23(10), 1245 (2007).

Evaluation of Behavioral Differences between Native Polycyclic Aromatic Hydrocarbons and 13C-Labeled Internal Standards during Clean-up Steps of Analysis
Nobuyasu ITOH, Masahiko NUMATA, and Takashi YARITA
National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan
We confirmed that concentrations of polycyclic aromatic hydrocarbons (PAHs) obtained by isotope dilution mass spectrometry (IDMS) using the corresponding 13C-labeled internal standards are reliable even after clean-up steps. Change in the ratio of phenanthrene to 13C6-phenanthrene was less than 0.2%, although the recovery yield of 13C6-phenanthrene decreased to 60%. Since changes in the ratios were commonly within the relative standard deviations of the concentrations (1.5 - 5.4%) obtained using gas chromatography-mass spectrometry in combination with pressurized-liquid extraction, concentrations obtained by IDMS with 13C-PAHs should be reliable.