Analytical Sciences

Abstract − Analytical Sciences, 22(5), 747 (2006).

Chiral Separation of Synthetic Vicinal Diol Compounds by Capillary Zone Electrophoresis with Borate Buffer and β-Cyclodextrin as Buffer Additive
Yan ZHAO,*Xingbin YANG,** Ru JIANG,* Xiaoli SUN,* Wenmin LIU,* and Shengyong ZHANG*
*Department of Chemistry, The Fourth Military Medical University, Xi'an 710032, China
**College of Life Science, Key Laboratory of Officinal Plant Resource and Pharmaceutical Chemistry, Ministry of Education, Shaanxi Normal University, Xi'an 710062, China
The investigation on capillary electrophoretic enantioseparation of six synthetic compounds containing vicinal diol groups has been undertaken to acquire the optimum conditions using native β-cyclodextrin (β-CD) as chiral selector and borate as a background electrolyte. The separation was carried out in an uncoated capillary (58.5 cm × 75 µm i.d., effective length 48.5 cm) and the effects of several important factors were investigated in detail. The results showed that β-CD as a chiral selector exhibited good enantioselectivity and that the enantioseparation was greatly influenced by the structure of the diols, the borate concentration and the buffer pH. The optimum performance was obtained for the chiral vicinal diols under the conditions of 200 mM borate buffer of pH 9.8 containing 1.7% β-CD at an applied voltage of 15 kV and a capillary temperature of 20°C. Under the conditions, four diols were baseline separated with fast analysis time and the good theoretical plate numbers (above 10 × 104) and favorable migration-time reproducibilities (RSDs below 3.0%) were obtained. The separation results were satisfactory.