Analytical Sciences

Abstract − Analytical Sciences, 22(5), 743 (2006).

Determination of Gatifloxacin and Ornidazole in Tablet Dosage Forms by High-Performance Thin-Layer Chromatography
Bhanubhai N. SUHAGIA, Shailesh A. SHAH, Ishwarsinh S. RATHOD, Haresh M. PATEL, Dinesh R. SHAH, and Bhavin P. MAROLIA
Department of Quality Assurance, L. M. College of Pharmacy, P. B. No. 4011, Navrangpura, Ahmedabad-380 009, Gujarat, India
A simple and sensitive high-performance thin-layer chromatography (HPTLC) method has been developed for the quantitative estimation of gatifloxacin and ornidazole in its combined dosage forms. Gatifloxacin and ornidazole were chromatographed on silica Gel 60 F254 TLC plate using n-butanol:methanol:ammonia (6 M) (8:1:1.5 v/v) as the mobile phase and scanned at 302 nm using a Camag TLC Scanner 3. The Rf value of gatifloxacin and ornidazole was found to be 0.21 ± 0.02 and 0.76 ± 0.04, respectively. The linearity of gatifloxacin and ornidazole were in the range of 100 - 500 ng/spot and 250 - 1250 ng/spot, respectively. The limit of detection was found to be 40 ng/spot for gatifloxacin and 100 ng/spot for ornidazole. The proposed method was applied for the determination of gatifloxacin and ornidazole in combined dosage forms.