Analytical Sciences

Abstract − Analytical Sciences, 22(12), 1515 (2006).

Interaction between Fluoroquinolones and Bovine Serum Albumin Studied by Affinity Capillary Electrophoresis
Shiwang LIU,* Li-Wei ZHANG,** and Xin-Xiang ZHANG**
*Department of Biology, Huanggang Normal University, Huanggang 438000, Hubei, China
**Beijing National Laboratory for Molecular Sciences (BNLMS), College of Chemistry, Peking University, Beijing 100871, China
The interactions between eight fluoroquinolone antibiotics (ciprofloxacin, enoxacin, fleroxacin, levofloxacin, lomefloxacin, norfloxacin, ofloxacin, pefloxacin) and bovine serum albumin (BSA) were studied by affinity capillary electrophoresis (ACE). The binding constants were estimated by the change of migration times of the analytes through the change of concentration of BSA in the buffer solution. The yield binding constants were between 3.19 × 104 and 1.21 × 105 M-1. These were related with the structures of fluoroquinolones, and agreed with the results obtained by other techniques. The obtained binding constants may help us in gaining some insights on possible drug/protein interactions and in early evaluation of the drugs' pharmacokinetic profiles during drug discovery.