Analytical Sciences

Abstract − Analytical Sciences, 21(9), 1117 (2005).

Fluorescence Analysis of p-Hydroxymethamphetamine in Urine by Thin-Layer Chromatography
Noriyuki KATO,* Hiroaki KUBO,** and Hiroshi HOMMA**
*Scientific Criminal Investigation Laboratory, Kanagawa Prefectural Police Headquarters, 155-1 Yamashita-cho, Naka-ku, Yokohama 231-0023, Japan
**School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
A highly-sensitivie analytical method for the detection of p-hydroxymethamphetamine (pOHMA) in urine is presented. The proposed method combines liquid-liquid extraction with acetonitrile and solid-phase extraction by thin-layer chromatography (TLC) with oxidation using potassium hexacyanoferrate(III) and sodium hydroxide to detect the fluorophor of pOHMA. The detection limit for pOHMA is 10 ng (n = 3). The analysis of pOHMA in forensic samples is successfully performed, without interference from endogenous fluorophors, yielding concentrations in the appropriate range for methamphetamine abusers.