Abstract − Analytical Sciences, 21(8), 997 (2005).
Determination of Antihypertensive Small Peptides, Val-Tyr and Ile-Val-Tyr, by Fluorometric High-Performance Liquid Chromatography Combined with a Double Heart-Cut Column-Switching Technique
Takao UENO, Mitsuru TANAKA, Toshiro MATSUI, and Kiyoshi MATSUMOTO
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan
A double column-switching HPLC method with naphthalene-2,3-dialdehyde (NDA) was applied for determination of two plasma antihypertensive peptides, Val-Tyr (VY) and Ile-Val-Tyr (IVY). After a first separation on a Phe-ODS column, double heart-cuts of the retention time corresponding to NDA-VY and NDA-IVY elutions were successfully separated on an analytical ODS column: 60% acetonitrile in 0.1% trifluoroacetic acid containing 5 mM sodium 1-octanesulfonate at 1.0 mL/min. Within-run coefficients of variation were 1.73% and 4.73% for VY and IVY, respectively.
J-STAGE:
View this article in J-STAGE
Back to the Table of Contents
