Analytical Sciences

Abstract − Analytical Sciences, 21(6), 709 (2005).

Spectrofluorometric Determination of Trace Amounts of Coenzyme II Using Norfloxacin-Terbium Complex as a Fluorescent Probe
Yusheng WANG, Jinkai LIU, and Chongqiu JIANG
Department of Chemistry, Shandong Normal University, Jinan, 250014, China
When terbium ion (Tb3+)-norfloxacin (NFLX) complex is issued a fluorescent probe, in a buffer solution of pH = 7.6, NADP can remarkably enhance the fluorescence intensity of the Tb3+-NFLX complex at λ = 545 nm. The enhanced fluorescence intensity of Tb3+ is in proportion to the concentration of NADP. The dynamic range for the determination of NADP is 1.11 × 10-7 - 6.16 × 10-5 mol l-1, with a detection limit of 4.31 × 10-8 mol l-1. This method is simple, practical and relatively free of interference from coexisting substances, so it can be successfully applied to determination of NADP in synthetic water samples.