Analytical Sciences

Abstract − Analytical Sciences, 21(6), 661 (2005).

A Simple Flow Injection Spectrophotometric Determination of Nitrite Based on Its Reaction with Thiourea
Mohammad POURHOSSEIN, Mohammad K. AMINI, and Morteza TALEBI
Chemistry Department, University of Isfahan, Isfahan, 81746-73441, Iran
A simple flow injection spectrophotometric method for the determination of nitrite is described. Nitrite injected into the flow system reacts with thiourea in acidic medium and the generated thiocyanate ion reacts with Fe(III) in the reagent solution to produce a highly colored product. The influences of chemical and physical parameters including reagent concentrations, sample volume injected, flow rates of the carrier and reagent solutions, reaction coil length and reaction temperature, were studied and optimum values of these parameters were established. Under the optimum conditions, the calibration curve for nitrite was linear over the concentration range 0.36 - 90 µg ml-1 without preconcentration and over the range 3.8 - 500 ng ml-1 with a simple online preconcentration step using an anion exchange column. The corresponding detection limits were 0.36 µg ml-1 and 3.8 ng ml-1, respectively. Up to 25 samples can be analyzed per hour, with an average relative standard deviation of ≤ 1.2%. Interferences by various foreign ions were studied and the method was applied to the determination of nitrite in water and spiked water samples.