Analytical Sciences

Abstract − Analytical Sciences, 21(4), 461 (2005).

Determination of Ambroxol in an Automated Multi-Pumping Pulsed Flow System
João L. M. SANTOS,* Arnaud CLAUSSE,** José L. F. C. LIMA,* M. L. M. F. S. SARAIVA,* and António O. S. RANGEL***
*REQUIMTE, Departamento de Química-Fisica, Faculdade de Farmácia, Universidade do Porto, Rua Anibal Cunha 164, 4050-047 Porto, Portugal
**Ecole Nationale Supérieure de Chimie de Clermont-Ferrand, 24 avenue des Landais, BP 187, 63174 Aubière Cedex, France
***Escola Superior de Biotecnologia, Universidade Católica Portuguesa Rua António Bernardino de Almeida, 4200-072 Porto, Portugal
A new flow methodology exploiting the multi-pumping approach was developed for the spectrophotometric determination of ambroxol hydrochloride in pharmaceutical preparations. The flow manifold was implemented by using, exclusively, multiple solenoid-actuated micro-pumps, which acted simultaneously as sample insertion, solutions propelling and reagents commutation units. Linear calibration plots were obtained over an ambroxol concentration ranging from 10 to 200 mg l-1 (r.s.d. < 0.5%, n = 15) and a sampling rate of about 60 samples per hour (flow rate = 1.92 ml min-1, sample volume = 80 µl).