Analytical Sciences

Abstract − Analytical Sciences, 21(4), 409 (2005).

Fluorometric Method for Quantitative Determination of Glucose and Its Application to Human Serum
Jigang GAO, Jie ZHOU, and Xiangjin QU
College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018 Shandong, People’s Republic of China
By coupling the Fenton reaction with the glucose oxidase (GOD)-catalyzed reaction, we have developed a fluorometric method for the determination of glucose; the linear response range and the detection limit are 0.108 - 2.59 µg/ml and 0.0432 µg/ml, respectively. This approach is selective and has the advantages of using inexpensive reagents and avoiding interferences. Satisfactory results were obtained for the determination of glucose in human serum samples.