Analytical Sciences

Abstract − Analytical Sciences, 21(4), 367 (2005).

An Amperometric Glucose Biosensor Based on Glucose Oxidase Immobilized in Electropolymerized Poly(o-aminophenol) and Carbon Nanotubes Composite Film on a Gold Electrode
Dawei PAN, Jinhua CHEN, Shouzhuo YAO, Wenyan TAO, and Lihua NIE
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China
An amperometric glucose biosensor is developed that is based on immobilization of glucose oxidase (GOD) in a composite film of poly(o-aminophenol) (POAP) and carbon nanotubes (CNT), which are electrochemically co-polymerized at a gold (Au) electrode. Because of the high surface per volume ratio and excellent electrical conductivity of CNT, the biosensor based on an Au/POAP/CNT/GOD electrode has lower detection limit (0.01 mM), larger maximum response current (0.24 mA cm-2) and higher sensitivity (11.4 mA M-1 cm-2) than the values of the biosensor based on an Au/POAP/GOD electrode. Additionally, the biosensor shows fast response time, large response current, and good anti-interferent ability for ascorbic acid, uric acid and acetaminophen. Good reproducibility and stability of the biosensor are also observed.