Analytical Sciences

Abstract − Analytical Sciences, 21(4), 349 (2005).

Surface Modification of Microchip Input Reservoirs for Pressure-induced Sample Injection into Microreactors
Vasily A. KAZAKOV,* Marina V. LAVROVA,* Alexander A. GANEEV,*,** Leonid N. MOSKVIN,* and Maxim N. SLYADNEV*,**
*Department of Chemistry, St. Petersburg State University, Petergoff, Universitetskij pr. 26, St. Petersburg, 198504, Russia
**Lumex Ltd., Moskovskij pr. 19, St. Petersburg, 190005, Russia
Optimization of geometry and surface modification of microchip input reservoirs were performed to achieve uninterferenced pressure-induced sample injection of multiple samples into microreactors using a single syringe pump. Nine samples of 3.5 µL were pipetted onto input reservoirs and loading of PCR mixture into 260 nL microreactors was achieved followed by successful PCR amplification, confirming that no cross-contamination occurs during injection.